EM map deposition questions
- How do I begin a map deposition to EMDB?
- What map formats are accepted by EMDB?
- What is the difference between a deposition session id and an EMDB accession id?
- We have two maps for one submitted manuscript, one native, one with cofactor protein. Do we need to complete two separate depositions?
- How can we deposit multiple 3D volumes generated from imaging of a single specimen?
- Our manuscript has not been officially accepted yet, so please do not release our entry.
- I received an email stating that my map is being released. When will it be available in the EM Data Bank?
- My structure has been published, how can I update the citation information for my entry?
- The journal I am targetting requires editors and referees access to the structure while my manuscript is under review. Does EMDB provide confidential access to editors or referees if a structure is "on hold"?
EM map-derived coordinate model deposition questions
- I have fitted coordinates for my map, can I include them with my map entry?
- I obtained coordinates from PDB and
used rigid-body fitting to place them into my
map. Can I deposit these coordinates to PDB?
- My fitted coordinates correspond to a protein homolog. During the PDB deposition, should I provide the species information/sequence information for the EM sample or for the protein homolog?
- How are coordinates derived from EM
- We have a new coordinate model fitted
into an EMDB map entry authored by another research
group. Can we deposit our coordinates?
EM map, model and metadata access questions
- How can I find/download a map or browse the EMDB database?
- How can I view EMDB maps?
- How can I download a map that has already been deposited and released?
- Can I use maps and/or map images from EMDB in my thesis/publication?
- How can I download EMDB map entry metadata?
EM map deposition answers
How do I begin a
map deposition to EMDB?
Beginning in 2016, EMDB maps can be deposited through the wwPDB Deposition & Annotation system. Please refer to our deposition page for details.
What map formats
are accepted by EMDB?
Maps can be deposited in mrc and ccp4 formats; they are converted to standard ccp4 format for redistribution in the EMDB.
What is the
difference between a deposition session id and an EMDB
accession id ?
When you initiate a deposition session you are assigned a session id (reference code). The session id is a "private" identifier known only to the depositor and the deposition site. When you complete your deposition and submit it to EMDB, you are assigned a public accession code. The public accession code is the id you should provide to journals when indicating your EMDB submission; it is also the code by which you can access to your entry in the publicly available EMDB search and atlas pages.
We have two maps
for one submitted manuscript, one native, one with
cofactor protein. Do we need to complete two separate
If the maps were generated from different specimen preparations/imaging experiments you will need to make two separate depositions. Time saving tip: complete the submission for the first map (deposition session 1), and then for the second map (deposition session 2) select the option "based on previous submission." You will need to provide the 1st deposition session id and password in order to use this option.
How can we deposit multiple
3D volumes generated from imaging of a single specimen?
EM reconstruction methods are beginning to yield ensembles of maps representing multiple conformations/enzymatic states within a heterogeneous population of imaged particles. If you need to deposit multiple maps of this type you can do so either within a single submission, in which case one map must be designated at the primary map, or a separate submissions (please see answer above).
Our manuscript has
not been officially accepted yet, so please do not
release our entry.
The only part of an EMDB submission that is released immediately is the experimental information in the header. The header information will only be released after you have had an opportunity to comment on the curated data. The map will not be released until the hold period has elapsed which can be on publication (HPUB), or 1 year from deposition. The map will only be released immediately if you have requested this at the release option stage.
I received an email
stating that my map is being released. When will it be
available in the EM Data Bank?
EMDB follows the same weekly release cycle as PDB. Maps queued for release before Thursday at 5pm (UK) will be available from EMDB on the following Wednesday.
The journal I am targetting
requires editors and referees access to the structure
while my manuscript is under review. Does EMDB provide
confidential access to editors or referees if a
structure is "on hold"?
We encourage you to deposit your maps and map-derived fitted models starting from the EMDataBank portal (http://emdatabank.org/deposit.html). Unreleased structural data on hold, both maps and coordinate sets, are kept strictly confidential. After deposition, you will immediately receive EMDB ids for your map entries and PDB ids for your map-derived model entries, which you may include in your manuscript. In addition, for PDB model entries you can obtain validation reports that provide assessments of model quality while keeping the coordinate file confidential. We encourage journal editors and referees to request validation reports from depositors as part of the manuscript submission and review process. The reports are date-stamped, and display the wwPDB processing site logo. For more information see http://www.wwpdb.org/validation.html.
EM map-derived coordinate model deposition answers
I obtained coordinates from
PDB and used rigid-body fitting to place them into my
map. Can I deposit these coordinates to PDB?
You can deposit coordinates derived from any fitting protocol, including rigid-body fitting, flexible fitting, or de novo tracing. The deposited coordinates must be aligned and in-frame with the map.
coordinates correspond to a protein homolog. During the
PDB deposition, should I provide the species
information/sequence information for the EM sample or
for the protein homolog?
The species information should correspond to your EM sample, but the sequence information should correspond to the protein homolog/fitted model.
How are coordinates derived
from EM reconstructions validated?
Currently, model fit to an EMDB map is validated through visual inspection of the deposited map + model pair using a 3D graphics program. Models are also checked for correct stereochemistry and clashes; a validation report is issued for every submitted model.
We have a new
coordinate model fitted into an EMDB map entry authored
by another research group. Can we deposit our
Yes, you can deposit your coordinates. Provide the EMDB map accession id (EMD-XXXX) as the associated EM volume of your coordinate deposition. The PDB policy regarding re-refined entries applies: "A re-refined entry may be deposited prior to publication but will not be processed (will have REFI status) or released until the associated publication has become publicly available." No changes will be made to the EMDB map entry.
EM map, model and metadata access answers
How can I
find/download a map or browse the EMDB archive?
Go to http://www.emdatabank.org/search.html and select either the UK or US EMSEARCH query page. You can search by id, author, title, release date, and more.
How can I view EMDB
We provide a java-based viewer on EMDataBank atlas pages, OpenAstexViewer, which allows the user to view the EMDB entry map at different contour levels and to also display any map-derived coordinate model PDB entries. To use the viewer, go to the visualization tab of the atlas page (as in this example) and click the "Launch" button. To view the map on your own computer, you will first need to download the map from the EMDB atlas page ("downloads" tab, see example), and view the map with locally installed software. UCSF Chimera, Pymol, Coot (v. 0.6.2 or later), and other graphics programs can display EMDB maps when loaded as "ccp4" format maps.
How can I download
a map that has already been deposited and released?
Released maps can be downloaded either from the 'DOWNLOAD' tab of the atlas page for the entry or from one of the three wwPDB ftp distribution sites: ftp://ftp.ebi.ac.uk/pub/databases/emdb, ftp://ftp.wwpdb.org/pub/emdb or ftp://pdb.protein.osaka-u.ac.jp/pub/emdb. Some strategies to retrieve files from the ftp sites are posted here: http://www.wwpdb.org/download/downloads.php
Can I use maps
and/or map images from EMDB in my thesis/publication?
Data files contained in the EMDB are free of all copyright restrictions and made fully and freely available for both non-commercial and commercial use. Users of the data should attribute the original authors of the structural data and the EMDB accession id. Molecular images created from 3D viewers on EMDataBank atlas pages are available under the same conditions. In that case, EMDataBank.org should also be cited (Lawson et al). Many of the static EM map images shown on atlas pages have been contributed by the original deposition authors in which case they may also be protected by copyright law, even though EMDataBank.org provides free access to them. The respective copyright holders of author-provided images retain rights for reproduction, redistribution and reuse. Users are responsible for compliance with copyright restrictions and are expected to adhere to the terms and conditions defined by the copyright holder.
How can I download
EMDB map entry metadata?
The EMDB is distributed as a branch of the wwPDB archive, and is available via all of the wwPDB ftp sites. Instructions to retrieve EMDB xml metadata files are posted here: http://www.wwpdb.org/download/downloads.php